メンバー紹介

名前: 樫田 啓 (かしだ ひろむ)
生年月日: 1979年10月22日
メールアドレス: kashida(at)chembio.nagoya-u.ac.jp
(アドレスご利用の際は(at)を@に変えてください)

プロフィール

1998.3 新潟県立新潟高等学校卒業
2002.3 東京大学工学部化学生命工学科卒業
2004.3 東京大学工学系研究科化学生命工学専攻修士課程修了
2004.4 東京大学工学系研究科化学生命工学専攻博士課程入学
2006.4 日本学術振興会特別研究員(DC2)
2006.9 東京大学工学系研究科化学生命工学専攻博士課程修了
博士(工学)

職歴

2006.10 日本学術振興会特別研究員(PD)
2007.4 名古屋大学大学院工学研究科 助教
2011.5 名古屋大学大学院工学研究科 講師
2013.2- 名古屋大学大学院工学研究科 准教授
2014.10
-2018.3
科学技術振興機構 さきがけ研究者(兼任)

研究題目

DNAと色素のコンジュゲーションによる新規機能性超分子の開発

主な業績

原著論文

  1. "Chaperone polymer-assisted, photo-driven DNA strand displacement"
    Cheng, B.; Kashida, H.; Shimada, N.; Maruyama, A.; Asanuma, H.
    ChemBioChem, 2017, 18, 1568-1572. DOI: 10.1002/cbic.201700394
    Selected as a "Front Cover"
  2. "Orientation-dependent FRET system reveals differences in structures and flexibilities of nicked and gapped DNA duplexes"
    Kashida, H.; Kurihara, A.; Kawai, H.; Asanuma, H.
    Nucleic Acids Res., 2017, 45(11), e105
  3. "Strand-invading linear probe combined with unmodified PNA"
    H. Aasanuma, R. Niwa, M. Akahane, K. Murayama, H. Kashida, Y. Kamiya
    Bioorg. Med. Chem., 2016, 24, 4129-4137. DOI:10.1016/j.bmc.2016.06.055
  4. "Visible Light-Triggered Crosslinking of DNA Duplex by Reversible [2+2] Photocycloaddition of Styrylpyrene"
    T. Doi, H. Kawai, K. Murayama, H. Kashida, H. Asanuma
    Chem. Eur. J., 2016, 22, 10533-10538
  5. "A stem-less probe using spontaneous pairing between Cy3 and quencher for RNA detection"
    H. Kashida, K. Morimoto, H. Asanuma
    Sci. Technol. Adv. Mater., 2016, 17, 267-273
  6. "Faint electric treatment-induced rapid endosomal escape for efficient delivery of functional macromolecules into the cytoplasm"
    M. Hasan, A. Nishimoto, T. Ohgita, S. Hama, H. Kashida, H. Asanuma, K. Kogure
    J. Control Release, 2016, 228, 20-25
  7. "Terminus-free siRNA prepared by photo-crosslinking activated via slicing by Ago2"
    Y. Kamiya, K. Iishiba, T. Doi, K. Tsuda, H. Kashida, H. Asanuma
    Biomater. Sci., 2015, 3, 1534-1538
    Selected as a "Back cover"
  8. "Hetero-Selective DNA-like Duplex Stabilized by Donor-Acceptor Interaction"
    T. Doi, T. Sakibara, H. Kashida, Y. Araki, T. Wada, H. Asanuma
    Chem. Eur. J., 2015, 21, 15974-15980. DOI:10.1002/chem.201502653
    Selected as a "Frontispiece"
  9. "Ultra-Sensitive Molecular Beacon Designed with Totally Serinol Nucleic Acid (SNA) for Monitoring mRNA in Cell"
    K. Murayama, Y. Kamiya, H. Kashida, H. Asanuma
    ChemBioChem, 2015, 16, 1298-1301, DOI: 10.1002/cbic.201500167
  10. "Molecular design of Cy3 derivative for highly sensitive in-stem molecular beacon and its application to the wash-free FISH"
    H. Kashida, T. Osawa, K. Morimoto, Y. Kamiya, H. Asanuma
    Bioorg. Med. Chem., 2015, 23, 1758-1762
  11. "Efficiency of [2+2] photodimerization of various stilbene derivatives within the DNA duplex scaffold"
    T. Doi, H. Kashida, H. Asanuma.
    Org. Biomol. Chem., 2015, 13, 4430-4437
    Selected as a "Front Cover"
  12. "Highly sensitive and robust linear probe for detection of mRNA in cells"
    H. Asanuma, M. Akahane, R. Niwa, H. Kashida, Y. Kamiya
    Angew. Chem. Int. Ed., 2015, 54, 4315-4319
  13. "Acyclic L-Threoninol Nucleic Acid (L-aTNA) with Suitable Structural Rigidity Cross-pairs with DNA and RNA."
    K. Murayama, H. Kashida, H. Asanuma
    Chem. Commun., 2015, 51, 6500-6503
    Selected as a "Back Cover"
  14. "Enhancement of stability and activity of siRNA by terminal substitution with Serinol Nucleic Acid (SNA)."
    Y. Kamiya, J. Takai, H. Ito, K. Murayama, H. Kashida, H. Asanuma
    ChemBioChem, 2014, 15, 2549-2555.
    Selected as a "Back Cover"
  15. "Selective labeling of mature RISC using siRNA carrying fluorophore-quencher pair."
    Y. Kamiya, A. Ito, H. Ito, M. Urushihara, J. Takai, T. Fujii, X.G. Liang, H. Kashida, H. Asanuma.
    Chem. Sci., 2013, 4, 4016-4021.
  16. "The “sugar-deficient” G-quadruplex: incorporation of aTNA in quadruplex structures."
    J. Zhou, K. Murayama, S. Amrane, H. Kashida, A. Bourdoncle, H. Asanuma, J. L. Mergny.
    Chem. Sci., 2013, 4, 3693-3698.
  17. "Highly stable duplex formation by artificial nucleic acids aTNA and SNA with acyclic scaffolds."
    K. Murayama, Y. Tanaka, T. Toda, H. Kashida, H. Asanuma.
    Chem. Eur. J., 2013, 19, 14151-14158.
  18. "p-Stilbazole moieties as artificial base pairs for photocrosslinking of DNA duplex."
    H. Kashida, T. Doi, T. Sakakibara, T. Hayashi, H. Asanuma.
    J. Am. Chem. Soc., 2013, 135, 7960-7966.
  19. "Evaluation of intrinsic spectroscopic properties of chromophore assemblies by shielding with cyclohexyl base pairs within a DNA duplex."
    H. Kashida, N. Higashiyama, T. Kato, H. Asanuma.
    Bioorg. Med. Chem., 2013, 21, 6191-6197.
  20. "Development of a robust model system of FRET using base-surrogates tethering fluorophores for strict control of their position and orientation within DNA duplex."
    T. Kato, H. Kashida, H. Kishida, H. Yada, H. Okamoto, H. Asanuma.
    J. Am. Chem. Soc., 2013, 135, 741-750.
  21. "Reversed assembling of dyes in RNA duplex compared with those in DNA"
    T. Fujii, M. Urushihara, H. Kashida, H. Ito, X.G. Liang, M. Yagi-Utsumi, K. Kato, H. Asanuma.
    Chem. Eur. J., 2012, 18, 13304-13313.
    Selected as a "frontispiece"
  22. "Quencher-free linear probe with multiple fluorophores on acyclic scaffold"
    H. Asanuma, M. Akahane, N. Kondo, T. Osawa, T. Kato, H. Kashida,
    Chem. Sci., 2012, 3, 3165-3169.
    Selected as an "Inside Back Cover"
  23. "Bulge-like asymmetric hetero dye-clustering in DNA duplex results in efficient quenching of background emission based on the maximized excitonic interaction"
    T. Fujii, Y. Hara, T. Osawa, H. Kashida, X.G. Liang, Y. Yoshida, H. Asanuma.
    Chem. Eur. J., 2012, 18, 10865-10872.
  24. "Quencher-free molecular beacon tethering 7-hydroxycoumarin detects targets through protonation/deprotonation"
    H. Kashida, K. Yamaguchi, Y. Hara, H. Asanuma.
    Bioorg. Med. Chem., 2012, 20, 4310-4315.
  25. "Preparation of supramolecular chromophoric assemblies using a DNA duplex" (Perspective)
    H. Kashida, H. Asanuma,
    Phys. Chem. Chem. Phys., 2012, 14, 7196-7204.
  26. "Design of an Artificial Functional Nanomaterial with High Recognition Ability"
    X.G. Liang, T. Mochizuki, T. Fujii, H. Kashida, H. Asanuma,
    Natural Computing, 2012, 11, 231-238.
  27. "Polycation-chaperoned in-stem molecular beacon system"
    H. Asanuma, T. Osawa, H. Kashida, T. Fujii, X.G. Liang, K. Niwa, Y. Yoshida, N. Shimada, A. Maruyama.
    Chem. Commun., 2012, 48, 1760-1762.
  28. "Cyclohexyl “base pairs” stabilize duplexes and intensify pyrene fluorescence by shielding it from natural base pairs."
    H. Kashida, K. Sekiguchi, N. Higashiyama, T. Kato, H. Asanuma.
    Org. Biomol. Chem., 2011, 9, 8313-8320.
  29. "Detection of three-base deletion by exciplex formation with perylene derivatives."
    H. Kashida, N. Kondo, K. Sekiguchi, H. Asanuma.
    Chem. Commun., 2011, 47, 6404-6406.
  30. "A Cationic dye triplet as a unique “glue” that can connect fully matched termini of DNA duplexes."
    H. Kashida, T. Hayashi, T. Fujii, H. Asanuma.
    Chem. Eur. J., 2011, 17, 2614-2622.
    Selected as a "VIP"
    Selected as an "Inside Cover"
  31. "Control of the Chirality and Helicity of Oligomers of Serinol Nucleic Acid (SNA) by Sequence Design."
    H. Kashida, K. Murayama, T. Toda, H. Asanuma.
    Angew. Chem. Int. Ed., 2011, 50, 1285-1288.
    Selected as a "Hot Paper"
  32. "Design of a Functional Nanomaterial with Recognition Ability for Constructing Light-Driven Nanodevices."
    X. G. Liang, T. Mochizuki, T. Fujii, H. Kashida, H. Asanuma.
    LNCS 2011, 6518, 112-122.
  33. "Unexpectedly stable artificial duplex from flexible acyclic threoninol."
    H. Asanuma, T. Toda, K. Murayama, X. G. Liang, H. Kashida.
    J. Am. Chem. Soc., 2010, 132, 14702-14703.
  34. "Insulator base pairs for lighting-up perylenediimide in a DNA duplex."
    H. Kashida, K. Sekiguchi, H. Asanuma.
    Chem. Eur. J., 2010, 16, 11554-11557.
  35. "Coherent Quenching of a Fluorophore for the Design of a Highly Sensitive In-Stem Molecular Beacon."
    Y. Hara, T. Fujii, H. Kashida, K. Sekiguchi, X. G. Liang, K. Niwa, T. Takase, Y. Yoshida, H. Asanuma.
    Angew. Chem. Int. Ed., 2010, 49, 5502-5506.
  36. "Accumulation of fluorophores into DNA duplexes to mimic the properties of quantum dots."
    H. Kashida, K. Sekiguchi, X. G. Liang, H. Asanuma.
    J. Am. Chem. Soc., 2010, 132, 6223-6230.
  37. "An efficient Fluorescent Resonance Energy Transfer (FRET) between pyrene and perylene assembled in a DNA duplex and its potential for discriminating single-base changes."
    H. Kashida, T. Takatsu, K. Sekiguchi, H. Asanuma.
    Chem. Eur. J., 2010, 16, 2479-2486.
  38. "Analysis of Coherent Heteroclustering of Different Dyes by Use of Threoninol Nucleotides for Comparison with the Molecular Exciton Theory."
    T. Fujii, H. Kashida, H. Asanuma.
    Chem. Eur. J., 2009, 15, 10092-10102.
  39. "In-Stem Molecular Beacon containing a Pseudo Base Pair of Threoninol Nucleotides for the Removal of Background Emission."
    H. Kashida, T. Takatsu, T. Fujii, K. Sekiguchi, X. G. Liang, K. Niwa, T. Takase, Y. Yoshida, H. Asanuma.
    Angew. Chem. Int. Ed., 2009, 48, 7044-7047.
  40. "Positively charged base surrogate for highly stable “base pairing”through electrostatic and stacking interactions."
    H. Kashida, H. Ito, T. Fujii, T. Hayashi, H. Asanuma.
    J. Am. Chem. Soc., 2009, 131, 9928-9930.
  41. "Modulation of pKa of Brooker's Merocyanine by DNA Hybridization."
    H. Kashida, K. Sano, Y. Hara, H. Asanuma.
    Bioconjugate Chem., 2009, 20, 258-265.
  42. "Postsynthetic modification of DNA via threoninol on a solid support by means of allylic protection."
    H. Asanuma, Y. Hara, A. Noguchi, K. Sano, H. Kashida.
    Tetrahedron Lett., 2008, 49, 5144-5146.
  43. "Threoninol as a Scaffold of Dyes (Threoninol-nucleotide) and Their Stable Interstrand Clustering in Duplexes."
    H. Kashida, T. Fujii, H. Asanuma.
    Org. Biomol. Chem., 2008, 6, 2892-2899.
  44. "Detection of genetic polymorphisms with high sensitivity by DNA-perylene conjugate."
    H. Kashida, T. Takatsu, H. Asanuma.
    Tetrahedron Lett., 2007, 48, 6759-6762.
  45. "2’,6’-Dimethylazobenzene as an efficient and thermo-stable photo-regulator for the photoregulation of DNA hybridization."
    H. Nishioka, X. G. Liang, H. Kashida, H. Asanuma.
    Chem. Commun., 2007, 4354-4356.
  46. "Enhancement of RNA cleavage activity of 10-23 DNAzyme by covalently introduced intercalator."
    H. Asanuma, H. Hayashi, J. Zhao, X. G. Liang, A. Yamazawa, T. Kuramochi, D. Matsunaga, Y. Aiba, H. Kashida, M. Komiyama.
    Chem. Commun., 2006, 5062-5064.
  47. "Exciplex Formation between Pyrene and N,N-Dimethylaniline in DNA for the Detection of One-base Deletion."
    H. Kashida, M. Komiyama, H. Asanuma.
    Chem. Lett., 2006, 35, 934-935.
  48. "Insertion of two pyrene moieties into oligodeoxyribonucleotides for the efficient detection of deletion polymorphisms."
    H. Kashida, H. Asanuma, M. Komiyama.
    Chem. Commun., 2006, 2768-2770.
    (Highlighted in Chemical Biology, B29, 1 (2006))
  49. "Covalent incorporation of methyl red dyes into double-stranded DNA for their ordered clustering."
    H. Kashida, M. Tanaka, S. Baba, T. Sakamoto, G. Kawai, H. Asanuma, M. Komiyama.
    Chem. Eur. J., 2006, 12, 777-784.
  50. "Alternating hetero H aggregation of different dyes by interstrand stacking from two DNA-dye conjugates."
    H. Kashida, H. Asanuma, M. Komiyama.
    Angew. Chem. Int. Ed., 2004, 43, 6522-6525.
  51. "Interstrand H-aggregation of cationic dyes for narrowing the absorption spectra and stabilizing the duplex."
    H. Kashida, H. Asanuma, M. Komiyama.
    Supramol. Chem., 2004, 16, 459-464.
  52. "NMR study on the photoresponsive DNA tethering an azobenzene. Assignment of the absolute configuration of two diastereomers and structure determination of their duplexes in the trans-form."
    X.G. Liang, H. Asanuma, H. Kashida, A. Takasu, T. Sakamoto, G. Kawai, M. Komiyama.
    J. Am. Chem. Soc., 2003, 125, 16408-16415.
  53. "DNA-Naphthyl Red conjugate as a visualizing probe of DNA hybridization."
    H. Asanuma, H. Kashida, X.G. Liang, M. Komiyama.
    Chem. Commun., 2003, 1536-1537.
  54. "DNA-Dye Conjugates for Controllable H* Aggregation."
    H. Asanuma, K. Shirasuka, T. Takarada, H. Kashida, M. Komiyama.
    J. Am. Chem. Soc., 2003, 125, 2217-2223.

国際学会Proceedingsなど

  1. "Efficient energy transfer from pyrene to perylene assembled inside DNA duplex."
    H. Kashida, T. Takatsu, H. Asanuma.
    Nucleic Acids Symp. Ser., 2009, 53, 29-30.
  2. "Incorporation of cationic dyes into DNA for distinct stabilization of duplex."
    H. Kashida, H. Itoh, T. Fujii, H. Asanuma.
    Nucleic Acids Symp. Ser., 2008, 52, 701-702.
  3. "Preparation of coherent hetero clusters with threoninol scaffold."
    T. Fujii, H. Kashida, H. Asanuma.
    Nucleic Acids Symp. Ser., 2008, 52, 699-700.
  4. "Construction of novel dye aggregates based on "comb-type" sequence."
    H. Kashida, T. Fujii, H. Asanuma.
    Nucleic Acids Symp. Ser., 2007, 51, 11-12.
  5. "Preparation of "comb-type" hetero aggregates by DNA-dye conjugation."
    T. Fujii, H. Kashida, H. Asanuma.
    Nucleic Acids Symp. Ser., 2007, 51, 277-278.
  6. "Development of high-sensitive DNA probe by using perylene."
    H. Kashida, T. Takatsu, H. Asanuma.
    Nucleic Acids Symp. Ser., 2007, 51, 279-280.
  7. "Incorporation of methyl group on azobenzene for the effective photo-regulation of hybridization and suppression of thermal isomerization."
    H. Nishioka, H. Kashida, M. Komiyama, X. G. Liang, H. Asanuma.
    Nucleic Acids Symp. Ser., 2006, 50, 85-86.
  8. "Development of a probe DNA which accompanies color change on hybridization."
    H. Kashida, H. Asanuma, M. Komiyama.
    Nucleic Acids Res. Suppl., 2003, 3, 143-144.

総説

  1. "生細胞内RNAイメージングのための人工核酸"
    樫田啓、浅沼浩之
    生体の科学, 2017, 68(5), 440-441
  2. "Development of pseudo base-pairs on D-threoninol which exhibit various functions."
    Kashida, H.; Asanuma, H.
    Bull. Chem. Soc. Jpn., 2017, 90, 475
  3. "DNA二重鎖による色素会合体の精密位置配向制御と光化学への展開"
    浅沼浩之、樫田啓
    固体物理, 2017, 52(3), 149-158
  4. "DNA二重鎖の高い安定性と直交性をもつ疑似塩基対の開発"
    樫田啓、浅沼浩之
    高分子論文集, 2017, 74(4)257-264.
  5. "Design of photo-functional oligonucleotide by "copolymerization" of natural nucleobases with base-surrogate prepared from acyclic scaffold."
    Asanuma, H.; Murayama, K.; Kamiya, Y.; Kashida, H.
    Polymer J., 2017, 49, 279-289
  6. "Acyclic artificial nucleic acids with phosphodiester bonds exhibit unique functions"
    H. Kashida, K. Murayama, H. Asanuma
    Polymer J., 2016, 48, 781-786
  7. "新規人工核酸SNAを用いたRNAイメージング"
    樫田啓、村山恵司、浅沼浩之
    生体の科学 2015, 66(2), 145-150
  8. "DNA二重鎖を足場とした配向依存的FRET"
    浅沼浩之、栗原綾子、樫田啓
    光化学 2014, 45, 146-147
  9. "De Novo Design of Functional Oligonucleotides with Acyclic Scaffolds"
    H. Asanuma, H. Kashida, Y. Kamiya
    Chem. Rec. 2014, 14, 1055-1069.
  10. "Coherent Interactions of Dyes Assembled on DNA"
    H. Asanuma, T. Fujii, T. Kato, H. Kashida.
    J. Photochem. Photobiol. C, 2012, 13, 124-135.
  11. "Rational design of functional DNA with a non-ribose acyclic scaffold"
    H. Kashida, X. G. Liang, H. Asanuma.
    Curr. Org. Chem., 2009, 13, 1065-1084.
  12. "第3の塩基対をつくる"
    樫田啓
    化学, 2009, 64, 70-71.
  13. "光機能性DNAの設計と応用"
    浅沼浩之、樫田啓
    高分子加工, 2004, 53(1), 37-43.

著書

  1. "遺伝子を蛍光検出するナノプローブ開発"
    浅沼浩之、樫田 啓
    "ナノテクノロジーが拓く未来の医療"、片岡一則 編著、2017年12月25日発行、丸善プラネット(株)
  2. "非環状骨格型人工核酸:aTNA, SNA"
    神谷由紀子、村山恵司、樫田 啓、浅沼浩之
    "核酸医薬の創製と応用展開"、監修:和田 猛、 p79-86、シーエムシー出版(2016)
  3. "Oligonucleotide Conjugates for Detection of Specific Nucleic Acid Sequences"
    H. Kashida, H. Asanuma
    "DNA Conjugates and Sensors", edited by K.R. Fox and T. Brown, RSC Publishing, 2012, pp.242-257.
  4. "人工核酸を利用したプローブDNAの調製と遺伝子多型検出"
    樫田啓、浅沼浩之
    "バイオチップ実用化ハンドブック"、監修:金子周一 堀池靖浩、pp.603-610、エヌ・ティー・エス(2010)

受賞歴

  • 若手科学者賞, 平成29年度科学技術分野の文部科学大臣表彰
  • 平成26年度高分子研究奨励賞, 公益社団法人高分子学会
  • 第63回進歩賞, 公益社団法人日本化学会, 2014年 
  • Outstanding Oral Presentation Award, The 40th International Symposium on Nucleic Acids Chemistry (Kanagawa Univ.), 2013年11月
  • 赤崎賞, 名古屋大学, 2012年3月
  • 講演賞, 第5回バイオ関連化学シンポジウム(つくば), 2011年9月
  • 若手研究者奨励講演賞, 第21回バイオ高分子研究会シンポジウム (関西大学), 2011年7月
  • Best Oral Presentation Award, XVth Symposium on Chemistry of Nucleic Acid Components (Czech Republic), 2011年6月
  • PCCP Prize, Royal Society of Chemistry, 2011年3月

趣味

スポーツ(Jリーグ、プロ野球、ラグビーなどなど)観戦、音楽鑑賞、読書、ソフトボール、旅